Atlantic bluefin tuna stomach contents 2018-2023, southern Gulf of St. Lawrence
PURPOSE:
This product serves a public facing webpage for the Canadian public to download Atlantic Bluefin Tuna stomach content data.
DESCRIPTION:
Metadata and stomach content from fish caught in the commercial fishery.
SAMPLING METHODS:
Stomachs were collected from Atlantic Bluefin Tuna (ABFT) caught from mid-August to late September over six years (2018–2023). Most samples originated from ABFT caught around the eastern end of Prince-Edward Island, which reflects the dominant ABFT fishing area, while a few samples were obtained from the Miscou/Baie-des-Chaleurs area in 2018 and 2019.
Fish were measured to the nearest curved fork length (cm) and weighed to the nearest round weight (kg). Stomachs were obtained directly from harvesters or through a fish buyer and were stored at −20 ◦C before being processed in the laboratory. Stomachs identification numbers were cross-referenced with ABFT tag numbers recorded by fish provider in order to obtain logbook and port data (catch location, time, weight length, sex, gear, etc.) for each sample. Stomachs were thawed in the laboratory and the content was sorted and identified to the lowest possible taxonomic level. For each stomach, prey were weighed collectively as a taxonomic group and individually to the nearest 0.1 g. Dead bait used to capture ABFT, identified by cut marks, were recorded and weighed but excluded from the analysis. Live bait items cannot be identified from stomach content analyses. Only a few otoliths were found in 2018 and their degraded quality precluded performing ageing or species identification. Rare and small prey items such as algae and rocks were classified in the category “other”. Fish remains that could not be identified were classified in the category “Unidentified teleostei remains”.
For 2019 to 2023, when stomach content items could not be visually identified and when tissue was available, tissue samples were collected and stored at −20 °C for DNA barcoding analysis. DNA extraction, mitochondrial cytochrome oxidase subunit 1 amplification, Sanger sequencing and species assignation were performed at the Plateforme d’Analyses Génomiques and Plateforme Bio-informatique of the Institut de Biologie Intégrative et des Systèmes (PAG-IBIS, Université Laval, Quebec city, QC, Canada, http://www.ibis.ulaval.ca/en/services-2/genomic-analysis-platform/). DNA was extracted from 20 mg of muscle tissue using the Omega Bio-tek E-Z-96 Tissue DNA Kit (Omega Bio-tek, Norcross GA, USA) following manufacturer instructions. The mitochondrial cytochrome oxidase subunit 1 region was amplified and sequenced as described in Hashemzadeh Segherloo et al., 2021). Sanger forward and reverse reads were analyzed independently using the Basic Local Alignment Search Tool against non-redundant sequences to identify the top hit for each sequence. When samples could not be identified by a top hit sequence they were classified as “unidentifiable fish”. Prey items that were successfully identified using DNA barcoding were incorporated into the stomach content analysis database and used in all subsequent diet analyses (abundance, occurrence and weight). The weight of the items used in the database was the weight of the remains as they were, and not reconstructed weights calculated for a live animal of the species identified by the barcoding.
USE LIMITATION:
To ensure scientific integrity and appropriate use of the data, we would encourage you to contact the data custodian.
Datasets available for download
Additional Info
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| Last Updated | October 20, 2025, 02:38 (UTC) |
| Created | October 20, 2025, 02:38 (UTC) |
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Domain / Topic
Domain or topic of the dataset being cataloged.
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Oceans |
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Title
Title for the Dataset.
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Atlantic bluefin tuna stomach contents 2018-2023, southern Gulf of St. Lawrence |
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Description
A description of the dataset.
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PURPOSE: This product serves a public facing webpage for the Canadian public to download Atlantic Bluefin Tuna stomach content data. DESCRIPTION: Metadata and stomach content from fish caught in the commercial fishery. SAMPLING METHODS: Stomachs were collected from Atlantic Bluefin Tuna (ABFT) caught from mid-August to late September over six years (2018–2023). Most samples originated from ABFT caught around the eastern end of Prince-Edward Island, which reflects the dominant ABFT fishing area, while a few samples were obtained from the Miscou/Baie-des-Chaleurs area in 2018 and 2019. Fish were measured to the nearest curved fork length (cm) and weighed to the nearest round weight (kg). Stomachs were obtained directly from harvesters or through a fish buyer and were stored at −20 ◦C before being processed in the laboratory. Stomachs identification numbers were cross-referenced with ABFT tag numbers recorded by fish provider in order to obtain logbook and port data (catch location, time, weight length, sex, gear, etc.) for each sample. Stomachs were thawed in the laboratory and the content was sorted and identified to the lowest possible taxonomic level. For each stomach, prey were weighed collectively as a taxonomic group and individually to the nearest 0.1 g. Dead bait used to capture ABFT, identified by cut marks, were recorded and weighed but excluded from the analysis. Live bait items cannot be identified from stomach content analyses. Only a few otoliths were found in 2018 and their degraded quality precluded performing ageing or species identification. Rare and small prey items such as algae and rocks were classified in the category “other”. Fish remains that could not be identified were classified in the category “Unidentified teleostei remains”. For 2019 to 2023, when stomach content items could not be visually identified and when tissue was available, tissue samples were collected and stored at −20 °C for DNA barcoding analysis. DNA extraction, mitochondrial cytochrome oxidase subunit 1 amplification, Sanger sequencing and species assignation were performed at the Plateforme d’Analyses Génomiques and Plateforme Bio-informatique of the Institut de Biologie Intégrative et des Systèmes (PAG-IBIS, Université Laval, Quebec city, QC, Canada, http://www.ibis.ulaval.ca/en/services-2/genomic-analysis-platform/). DNA was extracted from 20 mg of muscle tissue using the Omega Bio-tek E-Z-96 Tissue DNA Kit (Omega Bio-tek, Norcross GA, USA) following manufacturer instructions. The mitochondrial cytochrome oxidase subunit 1 region was amplified and sequenced as described in Hashemzadeh Segherloo et al., 2021). Sanger forward and reverse reads were analyzed independently using the Basic Local Alignment Search Tool against non-redundant sequences to identify the top hit for each sequence. When samples could not be identified by a top hit sequence they were classified as “unidentifiable fish”. Prey items that were successfully identified using DNA barcoding were incorporated into the stomach content analysis database and used in all subsequent diet analyses (abundance, occurrence and weight). The weight of the items used in the database was the weight of the remains as they were, and not reconstructed weights calculated for a live animal of the species identified by the barcoding. USE LIMITATION: To ensure scientific integrity and appropriate use of the data, we would encourage you to contact the data custodian. |
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Keywords/tags categorizing the dataset.
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Format (CSV, XLS, TXT, PDF, etc)
File format of the dataset.
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Dataset Size
Dataset size in megabytes.
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Metadata Identifier
Metadata identifier – can be used as the unique identifier for catalogue entry
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Published Date
Published date of the dataset.
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2025-02-27 |
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Time Period Data Span (start date)
Start date of the data in the dataset.
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Time Period Data Span (end date)
End date of time data in the dataset.
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GeoSpatial Area Data Span
A spatial region or named place the dataset covers.
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Access category
Type of access granted for the dataset (open, closed, service, etc).
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License
License used to access the dataset.
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Open Government Licence - Canada |
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Limits on use
Limits on use of data.
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Location
Location of the dataset.
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https://open.canada.ca/data/en/dataset/93b46916-f2e0-11ef-88e3-5d0a2a9fd0c5 |
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Data Service
Data service for accessing a dataset.
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Owner
Owner of the dataset.
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Fisheries and Oceans Canada | Pêches et Océans Canada |
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Contact Point
Who to contact regarding access?
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Government of Canada; Fisheries and Oceans Canada, [email protected] |
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Contact Point Email
The email to contact regarding access?
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Publisher
Publisher of the dataset.
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Publisher Email
Email of the publisher.
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[email protected] |
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Author
Author of the dataset.
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Author Email
Email of the author.
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Accessed At
Date the data and metadata was accessed.
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Identifier
Unique identifier for the dataset.
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Language
Language(s) of the dataset
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Link to dataset description
A URL to an external document describing the dataset.
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Persistent Identifier
Data is identified by a persistent identifier.
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Globally Unique Identifier
Data is identified by a persistent and globally unique identifier.
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Contains data about individuals
Does the data hold data about individuals?
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Contains data about identifiable individuals
Does the data hold identifiable data about individual?
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Contains Indigenous Data
Does the data hold data about Indigenous communities?
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Portal Type
Platform type of the source portal.
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Version
Version of the datatset
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None |
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Source
Source of the dataset.
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None |
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Version notes
Version notes about the dataset.
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Is version of another dataset
Link to dataset that it is a version of.
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Other versions
Link to datasets that are versions of it.
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Provenance Text
Provenance Text of the data.
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Provenance URL
Provenance URL of the data.
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Temporal resolution
Describes how granular the date/time data in the dataset is.
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GeoSpatial resolution in meters
Describes how granular (in meters) geospatial data is in the dataset.
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GeoSpatial resolution (in regions)
Describes how granular (in regions) geospatial data is in the dataset.
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Indigenous Community Permission
Who holds the Indigenous Community Permission. Who to contact regarding access to a dataset that has data about Indigenous communities.
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Community Permission
Community permission (who gave permission).
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The Indigenous communities the dataset is about
Indigenous communities from which data is derived.
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Number of data rows
If tabular dataset, total number of rows.
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Number of data columns
If tabular dataset, total number of unique columns.
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Number of data cells
If tabular dataset, total number of cells with data.
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Number of data relations
If RDF dataset, total number of triples.
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Number of entities
If RDF dataset, total number of entities.
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Number of data properties
If RDF dataset, total number of unique properties used by the triples.
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Data quality
Describes the quality of the data in the dataset.
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Metric for data quality
A metric used to measure the quality of the data, such as missing values or invalid formats.
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